The Autof ms1000 is a MALDI-TOF MS instrument developed in April 2018 that received CE-marked IVD clearance in June 2018. The EXS3000 is a MALDI-TOF MS instrument received CE-marked IVD clearance in October 2021. In the past few years, the mass spectrometry manufacturers are basically from Western countries, and in recent years, we began to see mass spectrometry from China on the market. It is worth mentioning that Antu Biological Company and Zybio Company are both in vitro diagnostic companies from China. 5, 6Ĭurrently, many companies are producing or selling commercial mass spectrometry for clinical microbial identification, such as: Brooker-Dalton, Biomeerier, Antu Biological, Zybio, and so on. Thus, this method has revolutionized microbial identification in clinical microbiology laboratories. Identifying microorganisms using MALDI-TOF MS is convenient, rapid, and accurate while also being cost-effective. 3, 4 This new application has proven useful in the diagnosis and treatment of infectious diseases that require a swift response. This method greatly promotes the development of microbial identification technology. 1, 2 As a new generation of microbial rapid identification and analysis technology, mass spectrometry is a method to detect proteins or nucleic acid ions with different mass-to-charge ratios after separation by the electric field. Over the past two decades, Laboratory diagnosis of melioidosis has shifted to PCR-based sensitive detection methods such as gene sequencing and qPCR, and rapid specific identification techniques such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Keywords: evaluation, MALDI-TOF mass spectrometry, EXS3000, Autof ms1000, clinical isolates, identification, gram-negative bacteria, gram-positive bacteria, mycobacteria, yeast, anaerobic bacteria It’s worth mentioning that the target plate of the EXS 3000 instrument is reusable, but the target plate of the Autof ms1000 is disposable, making the EXS3000 more effective in reducing costs. The correct results at species level of the Autof ms1000 were 92.86% (65/70) for gram-negative bacteria, 91.38% (53/58) for gram-positive cocci, 93.94% (31/33) for yeast, 100% (15/15) for anaerobes and 78.79% (26/33) for filamentous fungi.Ĭonclusion: Although the results show that the EXS3000 and Autof ms1000 systems are equally good choices in terms of analytical efficiency for routine procedures, the test result of EXS3000 is slightly better than Autof ms1000. Genus-level discrimination was 205 (98.09%) by the EXS3000 and 205 (98.09%) by the Autof ms1000, respectively. Results: Current study found that species-level discrimination was found to be 191 (91.39%) and 190 (90.91%) by EXS3000 and Autof ms1000, respectively. Sequence analysis of 16S rRNA or ITS regions was used to verify all strains. All strains were identified by EXS3000 (Zybio, China) and Autof ms1000 (Autobio, China). Methods: A total of 209 common clinical common isolates, including 70 gram-negative bacteria strains, 58 gram-positive bacteria strains, 33 yeast strains, 15 anaerobic bacteria strains, and 33 mold strains, and 19 mycobacterial strains were tested. Here, we reported the performance of two MALDI-TOF MS instruments, EXS3000 (Zybio, China) and Autof ms1000 (Autobio, China), which are commonly used in clinical microbiology field. Purpose: Matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF) has recently been widely used in clinical microbiology laboratories, with the advantages of being reliable, rapid, and cost-effective. 83 Feishan Street, Yunyan District, Guiyang City, Guizhou Province, People’s Republic of China, Tel +86-13658504875, Email 1800 Yuntai Road, Pudong New Area, Shanghai, People’s Republic of China, Tel +86-17701810639, Email Chunying Yang, Department of Laboratory Medicine, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, No. *These authors contributed equally to this workĬorrespondence: Jian Guo, Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, No. Lijuan Xiong, 1, * Xu Long, 1, * Lijun Ni, 2, * Lili Wang, 2 Yang Zhang, 1 Lili Cui, 1 Jian Guo, 2 Chunying Yang 1ġDepartment of Laboratory Medicine, the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guizhou, People’s Republic of China 2Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China
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